abstract：:It is established that high concentrations of nitric oxide(1) (NO), as released from activated macrophages, induce apoptosis in breast cancer cells. In this study, we assessed the potential of a light-activated NO donor [(Me2bpb)Ru(NO)(Resf)], a recently reported apoptototic agent, in suppressing the anchorage indepen...

journal_title：Archives of biochemistry and biophysics

authors： Sen S,Kawahara B,Fry NL,Farias-Eisner R,Zhang D,Mascharak PK,Chaudhuri G

更新日期：2013-12-01 00:00:00

abstract：:Resonance Raman spectroscopy (RRS) is a non-invasive method that has been developed to assess carotenoid status in human tissues including human skin in vivo. Skin carotenoid status has been suggested as a promising biomarker for human studies. This manuscript describes research done relevant to the development of thi...

journal_title：Archives of biochemistry and biophysics

authors： Mayne ST,Cartmel B,Scarmo S,Jahns L,Ermakov IV,Gellermann W

更新日期：2013-11-15 00:00:00

abstract：:Recent analyses of the orange, red, and purple plumages of cotingas (Cotingidae) and broadbills (Eurylaimidae) revealed the presence of novel carotenoid molecules, suggesting that the diversity of pigments and the metabolic transformations they undergo are not yet fully understood. Two Old World orioles, the Black-and...

journal_title：Archives of biochemistry and biophysics

authors： LaFountain AM,Frank HA,Prum RO

更新日期：2013-11-15 00:00:00

abstract：:G protein-coupled receptors can be directly modulated by changes in transmembrane voltage in a variety of cell types. Here we show that, while changes in the membrane voltage itself do not induce detectable modifications in the cytosolic Ca(2+) concentration, platelet stimulation with thrombin or the PAR-1 and PAR-4 a...

journal_title：Archives of biochemistry and biophysics

authors： Albarrán L,Dionisio N,López E,Salido GM,Rosado JA

更新日期：2013-10-15 00:00:00

abstract：:Lipid homeostasis in liver is known to be altered with diabetes mellitus, ultimately leading to liver damage and related complications. The present work aimed to evaluate changes in the liver phospholipid profile after 4 months of uncontrolled hyperglycemia. Twenty Wistar rats were divided into two groups: control and...

journal_title：Archives of biochemistry and biophysics

authors： Simões C,Domingues P,Ferreira R,Amado F,Duarte JA,Vitorino R,Neuparth MJ,Nunes C,Rocha C,Duarte I,Domingues MR

更新日期：2013-10-15 00:00:00

abstract：:We studied the effect of KAR-2 on cytosolic Ca(2+) level in human neutrophils by using a fluorescent dye (Fura-2) trapped in the cells. KAR-2 is a semisynthetic bis-indole derivative that shares vinblastine anti-microtubular properties, but does not share the vinblastine antagonistic effect on calmodulin. Therefore KA...

journal_title：Archives of biochemistry and biophysics

authors： Salerno C,Capuozzo E

更新日期：2013-09-01 00:00:00

abstract：:Methylibium petroleiphilum strain PM1 uses various petroleum products including the fuel additive methyl tert-butyl ether and straight chain and aromatic hydrocarbons as sole carbon and energy sources. It has two operons, dmpI and dmpII, that code for the enzymes in a pair of parallel meta-fission pathways. In order t...

journal_title：Archives of biochemistry and biophysics

authors： Terrell CR,Burks EA,Whitman CP,Hoffman DW

更新日期：2013-09-01 00:00:00

abstract：:Hyperammonemia is a major pathophysiological factor in encephalopathies associated with acute and chronic liver failure. On mouse brain slice preparations we analyzed the effects of ammonium on the characteristics of corticostriatal long-term potentiation (LTP) induced by high-frequency electrical stimulation (HFS) of...

journal_title：Archives of biochemistry and biophysics

authors： Chepkova AN,Sergeeva OA,Haas HL

更新日期：2013-08-15 00:00:00

abstract：:The effect of the nemaline myopathy-causing E117K mutation in β-tropomyosin (TM) on the structure and function of this regulatory protein was studied. The E117K mutant was found to have indistinguishable actin affinity compared with wild-type (WT) and similar secondary structure as measured by circular dichroism. Howe...

journal_title：Archives of biochemistry and biophysics

authors： Karpicheva OE,Robinson P,Piers A,Borovikov YS,Redwood CS

更新日期：2013-08-01 00:00:00

abstract：:Previous experiments performed in recombinant systems have suggested that protein-protein interactions occur between the UGTs and may play a significant role in modulating enzyme activity. However, evidence of UGT protein-protein interactions either in vivo or in more physiologically relevant in vitro systems has yet ...

journal_title：Archives of biochemistry and biophysics

authors： Konopnicki CM,Dickmann LJ,Tracy JM,Tukey RH,Wienkers LC,Foti RS

更新日期：2013-07-15 00:00:00

abstract：:Transforming growth factor-β (TGF-β) is pivotal in the pathogenesis of diabetic nephropathy. Type 1 TGF-β receptor (TGF-βR1) is degraded by Smad7-dependent ubiquitination-proteasomal pathway, which is deubiquitinated by ubiquitin C-terminal hydrolase-L5 (UCHL5). Therefore, we studied the role of UCHL5 in high glucose ...

journal_title：Archives of biochemistry and biophysics

authors： Ko YM,Chang CY,Chiou SJ,Hsu FJ,Huang JS,Yang YL,Guh JY,Chuang LY

更新日期：2013-07-15 00:00:00

abstract：:The enzyme phenylalanine hydroxylase catalyzes the hydroxylation of excess phenylalanine in the liver to tyrosine. The enzyme is regulated allosterically by phenylalanine and by phosphorylation of Ser16. Hydrogen/deuterium exchange monitored by mass spectrometry has been used to gain insight into any structural change...

journal_title：Archives of biochemistry and biophysics

authors： Li J,Fitzpatrick PF

更新日期：2013-07-15 00:00:00

abstract：:Mucus hypersecretion is a common feature in chronic airway diseases, and serine proteases play a critical role in this process. However, the mechanisms by which serine proteases induce mucin5AC hypersecretion have not been fully explored. In this study, we characterized human airway trypsin-like protease (HAT), a seri...

journal_title：Archives of biochemistry and biophysics

authors： Liu C,Li Q,Zhou X,Kolosov VP,Perelman JM

更新日期：2013-07-15 00:00:00

abstract：:ATP-activated P2Y receptors play an important role in renal sodium excretion. The aim of this study was to evaluate the modulation of ATPase-driven sodium reabsorption in the proximal tubule by ATP or adenosine (Ado). LLC-PK1 cells, a model of porcine proximal tubule cells, were used. ATP (10(-6)M) or Ado (10(-6)M) sp...

journal_title：Archives of biochemistry and biophysics

authors： Wengert M,Ribeiro MC,Abreu TP,Coutinho-Silva R,Leão-Ferreira LR,Pinheiro AA,Caruso-Neves C

更新日期：2013-07-15 00:00:00

abstract：:The phosphorylation state of several cardiac myofilament proteins changes with the level of stretch in intact, twitch-contracting cardiac muscles. It remains unclear which kinases are involved in the length-dependent phosphorylation of these proteins. We set out to investigate which kinases are involved after a step-w...

journal_title：Archives of biochemistry and biophysics

authors： Monasky MM,Taglieri DM,Jacobson AK,Haizlip KM,Solaro RJ,Janssen PM

更新日期：2013-07-01 00:00:00

abstract：:The Xin repeat-containing proteins, Xinα (Xirp1) and Xinβ (Xirp2), localize to the intercalated discs (ICDs) of mammalian hearts. Mouse Xinα (mXinα) directly interacts with β-catenin and actin filaments, potentially coupling the N-cadherin/β-catenin complexes to the underlying actin cytoskeleton and modulating ICD int...

journal_title：Archives of biochemistry and biophysics

authors： Wang Q,Lu TL,Adams E,Lin JL,Lin JJ

更新日期：2013-07-01 00:00:00

abstract：:Neural stem and progenitor cells (NSCs and NPs) give rise to the central nervous system (CNS) during embryonic development. NSCs and NPs differentiate into three main cell-types of the CNS; astrocytes, oligodendrocytes, and neurons. NSCs are present in the adult CNS and are important in maintenance and repair. Adult N...

journal_title：Archives of biochemistry and biophysics

authors： Ramasamy S,Narayanan G,Sankaran S,Yu YH,Ahmed S

更新日期：2013-06-01 00:00:00

abstract：:Stem cells maintain their self-renewal and multipotency capacities through a self-organizing network of transcription factors and intracellular pathways activated by extracellular signaling from the microenvironment or "niche" in which they reside in vivo. In the adult mammalian brain new neurons continue to be genera...

journal_title：Archives of biochemistry and biophysics

authors： Porlan E,Perez-Villalba A,Delgado AC,Ferrón SR

更新日期：2013-06-01 00:00:00

abstract：:The study aimed to evaluate the impact of adenosine receptors (ARs) on human colon tumor cells (HCT 116, HT-29) growth and sensitivity to 5-Fluorouracil (5-FU) an anticancer chemotherapeutic drug. The exposure of cancer cells to a selective A(3)-AR agonist (IB-MECA) resulted in an increase in HT-29 cells number, where...

journal_title：Archives of biochemistry and biophysics

authors： Sakowicz-Burkiewicz M,Kitowska A,Grden M,Maciejewska I,Szutowicz A,Pawelczyk T

更新日期：2013-05-01 00:00:00

abstract：:Cytochrome P4501B1 (Cyp1b1) is expressed specifically in certain neural crest (NC) cells during embryogenesis. Mesenchymal progenitor cells that develop from NC cells are modeled here by mouse C3H10T1/2 and 3T3-L1 cells. Dexamethasone in combination with methylisobutylxanthine (DM) induces Cyp1b1 and a 6.7 kb mouse Cy...

journal_title：Archives of biochemistry and biophysics

authors： Zheng W,Tong T,Lee J,Liu X,Marcus C,Jefcoate CR

更新日期：2013-04-01 00:00:00

abstract：:We previously showed that knockdown of the anaplerotic enzyme pyruvate carboxylase in the INS-1 832/13 insulinoma cell line inhibited glucose-stimulated insulin release and glucose carbon incorporation into lipids. We now show that knockdown of fatty acid synthase (FAS) mRNA and protein also inhibits glucose-stimulate...

journal_title：Archives of biochemistry and biophysics

authors： MacDonald MJ,Hasan NM,Dobrzyn A,Stoker SW,Ntambi JM,Liu X,Sampath H

更新日期：2013-04-01 00:00:00

abstract：:In this study, the binding points of MethB and two structurally-related cationic phenoxazine dyes [meldola blue (MB) and nile blue (NB)] to human butyrylcholinesterase (BChE) were investigated by molecular docking and site directed mutagenesis. The comparative inhibitory effects of MethB, MB and NB on recombinant wild...

journal_title：Archives of biochemistry and biophysics

authors： Sezgin Z,Biberoglu K,Chupakhin V,Makhaeva GF,Tacal O

更新日期：2013-04-01 00:00:00

abstract：:Differential scanning calorimetry measures the heat capacity of states and the excess heat associated with transitions that can be induced by temperature change. The integral of the excess heat capacity is the enthalpy for this process. Despite this potentially intimidating sounding physical chemistry background, DSC ...

journal_title：Archives of biochemistry and biophysics

authors： Johnson CM

更新日期：2013-03-01 00:00:00

abstract：:The nature and role of intermediates have been the subject of much heated debate in the field of protein folding. Historically, intermediates were viewed as essential stepping-stones that guide a protein through the folding process to the native state. However, with the experimental identification of numerous small pr...

journal_title：Archives of biochemistry and biophysics

authors： Tsytlonok M,Itzhaki LS

更新日期：2013-03-01 00:00:00

abstract：:The common notion in the protein world holds that proteins are synthesized as a linear polypeptide chain, followed by folding into a unique, functional 3D-structure. As outlined in many articles of this volume, this is in fact the case for a great proportion of the proteome. Many proteins and protein domains, however,...

journal_title：Archives of biochemistry and biophysics

authors： Kovacs D,Szabo B,Pancsa R,Tompa P

更新日期：2013-03-01 00:00:00

abstract：:The cellulose synthase protein (AcsAB) is encoded by a single gene in Gluconacetobacter hansenii ATCC 23769. We have examined the processing pattern of this enzyme and the localization of the cleavage products by heterologously expressing the truncated portions of the AcsAB protein and using specific antibodies genera...

journal_title：Archives of biochemistry and biophysics

authors： Iyer PR,Liu YA,Deng Y,McManus JB,Kao TH,Tien M

更新日期：2013-01-15 00:00:00

abstract：:In this study we related metacarb (N-(2-(3,5-bis(dimethylcarbamoyloxy)phenyl)-2-hydroxyethyl)propan-2-aminium chloride) and isocarb (N-(2-(3,4-bis(dimethylcarbamoyloxy)phenyl)-2-hydroxyethyl)propan-2-aminium chloride) inhibition selectivity, as well as stereoselectivity of mouse acetylcholinesterase (AChE; 3.1.1.7) an...

journal_title：Archives of biochemistry and biophysics

authors： Bosak A,Smilović IG,Stimac A,Vinković V,Sinko G,Kovarik Z

更新日期：2013-01-15 00:00:00

abstract：:Autologous venous grafts, used to circumvent occluded coronary arteries during coronary artery bypass, often develop thrombosis and neointimal hyperplasia. During neointimal hyperplasia, vascular smooth muscle cells (VSMCs), exposed to substantially higher pressure and hemodynamic forces, proliferate and extracellular...

journal_title：Archives of biochemistry and biophysics

authors： Song L,Duan P,Guo P,Li D,Li S,Xu Y,Zhou Q

更新日期：2012-12-15 00:00:00

abstract：:The putrescine analogue 1,4-diamino-2-butanone (DAB) is highly toxic to various microorganisms, including Trypanosoma cruzi. Similar to other α-aminocarbonyl metabolites, DAB exhibits pro-oxidant properties. DAB undergoes metal-catalyzed oxidation yielding H(2)O(2), NH(4)(+) ion, and a highly toxic α-oxoaldehyde. In v...

journal_title：Archives of biochemistry and biophysics

authors： Soares CO,Colli W,Bechara EJ,Alves MJ

更新日期：2012-12-15 00:00:00

abstract：:Barley α-amylase isozyme 1 (AMY1, EC 3.2.1.1) contains two surface binding sites, SBS1 and SBS2, involved in the degradation of starch granules. The distinct role of SBS1 and SBS2 remains to be fully understood. Mutational analysis of Tyr-380 situated at SBS2 previously revealed that Tyr-380 is required for binding of...

journal_title：Archives of biochemistry and biophysics

authors： Nielsen JW,Kramhøft B,Bozonnet S,Abou Hachem M,Stipp SL,Svensson B,Willemoës M

更新日期：2012-12-01 00:00:00

abstract：:While the general catalytic mechanism of the widespread serine hydrolase superfamily has been documented extensively, much less is known about its varied modes of functional modulation within biological systems. Under oxidizing conditions, inhibition of Saccharomyces cerevisiae S-formylglutathione hydrolase (SFGH, hom...

journal_title：Archives of biochemistry and biophysics

authors： Legler PM,Leary DH,Hervey WJ 4th,Millard CB

更新日期：2012-12-01 00:00:00

abstract：:Differentiation of pluripotent and lineage restricted stem cells such as neural stem cells (NSCs) was studied on conducting substrates of various nature without perturbation of the genome with exogenous genetic material or chemical stimuli. Primary mouse adult neural stem cells (NSCs) and P19 pluripotent embryonal (P1...

journal_title：Archives of biochemistry and biophysics

authors： Ostrakhovitch EA,Byers JC,O'Neil KD,Semenikhin OA

更新日期：2012-12-01 00:00:00

abstract：:Diets with high content of antioxidant polyphenols are associated with low prevalence of cardiovascular diseases and cancer. Inflammatory angiogenesis is a key pathogenic process both in cancer and atherosclerosis, and is tightly regulated by the proinflammatory enzyme cyclooxygenase (COX)-2 and the matrix degrading e...

journal_title：Archives of biochemistry and biophysics

authors： Scoditti E,Calabriso N,Massaro M,Pellegrino M,Storelli C,Martines G,De Caterina R,Carluccio MA

更新日期：2012-11-15 00:00:00

abstract：:The remarkable success of therapeutic applications of immunoglobulin G (IgG) in form of monoclonal antibodies and pooled immunoglobulin G preparations has directed attention to this class of glycoproteins. It is commonly appreciated that oligosaccharides attached to the Fc-region play a critical role in the biological...

journal_title：Archives of biochemistry and biophysics

authors： Loos A,Steinkellner H

更新日期：2012-10-15 00:00:00

abstract：:The enzyme catalase catalyzes the breakdown of hydrogen peroxide into oxygen and water. It is the main regulator of hydrogen peroxide metabolism. Hydrogen peroxide is a highly reactive small molecule formed as a natural byproducts of energy metabolism. Excessive concentrations may cause significant damages to protein,...

journal_title：Archives of biochemistry and biophysics

authors： Góth L,Nagy T

更新日期：2012-09-15 00:00:00

abstract：:Small compounds that activate the insulin-dependent signaling pathway have potential therapeutic applications in controlling type 2 diabetes mellitus. The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is an insulin-inducible transcription factor that decreases expression of the phosphoenolpyruvate carbo...

journal_title：Archives of biochemistry and biophysics

authors： Haneishi A,Takagi K,Asano K,Yamamoto T,Tanaka T,Nakamura S,Noguchi T,Yamada K

更新日期：2012-09-01 00:00:00

abstract：:Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to the immunoglobulin superfamily and contribute to cell-cell adhesion and signal modulation in various tissues. In humans, several CEACAMs are targeted by pathogenic bacteria. One peculiar member of this family, CEACAM3, is exclusively expresse...

journal_title：Archives of biochemistry and biophysics

authors： Buntru A,Roth A,Nyffenegger-Jann NJ,Hauck CR

更新日期：2012-08-01 00:00:00

abstract：:The hnRNP A/B family contains abundant nuclear proteins with major roles in alternative splicing and the ability for nucleo-cytoplasmic shuttling. Compared to the best known members of this family (hnRNP A1, A2/B1), hnRNP A3 is a relatively less known protein. We report herein immunochemical studies with the hnRNP A3 ...

journal_title：Archives of biochemistry and biophysics

authors： Papadopoulou C,Boukakis G,Ganou V,Patrinou-Georgoula M,Guialis A

更新日期：2012-07-15 00:00:00

abstract：:Vitamin D was discovered as an anti-rachitic agent, but even at present, there is no direct evidence to support the concept that vitamin D directly stimulates osteoblastic bone formation and mineralization. It appears to be paradoxical, but vitamin D functions in the process of osteoclastic bone resorption. In 1952, C...

journal_title：Archives of biochemistry and biophysics

authors： Suda T,Takahashi F,Takahashi N

更新日期：2012-07-01 00:00:00

abstract：:The vitamin D receptor (VDR) is a member of the nuclear receptor superfamily and plays a central role in the biological actions of vitamin D. VDR regulates the expression of numerous genes involved in calcium/phosphate homeostasis, cellular proliferation and differentiation, and immune response, largely in a ligand-de...

journal_title：Archives of biochemistry and biophysics

authors： Wang Y,Zhu J,DeLuca HF

更新日期：2012-07-01 00:00:00